Ribotyping Analysis of Microbes Producing Hydrolase from Batik Liquid Waste Samples

Abstract

Batik liquid waste that is disposed of into the environment still contains high levels of organic compounds as residue from the main ingredients in batik production, like dyes, wax, starch, soda ash, and others. With its high organic material content, batik liquid waste is a potential habitat for microbial growth. This research aims to isolate and genetically analyze microbes in batik liquid waste, namely by ribotyping analysis of microbes that produce hydrolase enzymes. From the results of microbial screening on selective lipase media, eight types of bacteria were obtained which have the potential to produce hydrolase enzymes. Eight types of hydrolase-producing bacteria have been genetically identified using the 16S rRNA gene. Alignment analysis using blastN from NCBI of the 16S rRNA gene nucleotide sequence of the sample suggested that the hydrolase-producing microbial isolate of the sample had the closest homology to the Serratia, Burkholderia, Stutzerimonas, and Pseudomonas groups. Four of the eight samples, RR1R, RA1R, RA2R, and ZB16R, have the highest homology <97%. This suggests that the four samples are novel microbial variants, which are different from the microbial data in Genbank.